Mid Term Molecular Biology Laboratory Exam King Abdulaziz university

Contents

Mid Term Molecular Biology

Sample From The exam::

Give a reason :
1. In detection of Monosaccharides experiment we add 5 drops of HCl to sucrose solution:
…………………… to hydolyse sucrose into glucose and fructose
2. The secondary structure of the protein:
……….It results from Hydrogen (H)-bonding of the poly-peptide chain………..……

Give a reason :
1. Formation of the Globular protein that dissolves in water.
There are 3 main types of chemical bonds that contributes to the formation of tertiary structure:
H-bond, Ionic bond and disulfide bond (-S-S-), which result in formation of a Globular protein
that dissolves in water (soluble)
2. We use yeast in Sucrose experiment:
To Prepare invertase enzyme extract by grinding 10 gm of yeast with 5 ml distilled water
and table sugar………